技术文章Article 首 页 > 技术文章 > 常见培养基配方
常见培养基配方
点击次数：4727 更新时间：2015-07-28
 

主要培养基的种类与组成

一.White培养基 二、LS培养基RM-64 三.HB培养基

四．MS培养基RM—62 五.花肥培养基 六．KC培养基

七.Kassanis培养基 八.木本植物用培养基 九.Nitsch&Nitsch(1969)培养基

十.Nielsen(1960)培养基 十一.MT(murashgeandTucker)培养基 十二.Miller培养基(1963)

十三.B5培养基 十四.N6培养基 十五.H培养基

十六.改良White培养基

锚点一.White培养基,White(1963)

1.无机盐类

Ca(NO3)2·4H2O287mg/L

KNO380mg/L

KCl65mg/L

NaH2PO4·H2O19.1mg/L

MgSO4·7H2O738mg/L

Na2SO4：·10H2O453mg/L

MnSO4·4H2O6.6mg/L

H3BO31.5mg/L

ZnSO4·7H2O2.7mg/L

KI0.75mg/L

2．有机物

甘氨酸3.0mg/L

烟酸0.5mg/L

维生素B60.1mg/L

维生素B10.1mg/L

柠檬酸2.0mg/L

蔗糖20g/L

pH5．7

锚点二、LS培养基RM-64[Linsmair&SKoog(1964))]

1．无机盐

NH4N03l650mg／L

K900mg/L

CaCl2·2H2O440mg/L

MgSO4·7H2O370mg/L

KH2PO4170mg/L

Na2一EDTA37.3mg/L

FeSO4·7H2O27.8mg/L

H3BO36.2mg/L

MnSO4·4H2O22.3mg/L

ZnSO4·7H2O8.6mg/L

KI0.83mg/L

Na2MoO·2H2O0.25mg/L

CuSO4·5H2O0.025mg/L

CoCl2·6H2O0.025mg/L

2．有机物

维生素Bl0．4mg/L

肌醇10．0mg／L

蔗糖30g／L

pH5．6

*FeSO4·7H2O5．57g，Na2一EDTA7．45g

先溶于1升蒸馏水里，然后按每升培养基含

5mg的比例加入培养基中。


锚点三.HB培养基Holley&Baker(1963)

1.无机盐

(以1/2浓度的Knop液为基础)

Ca(NO3)2.4H2O1000mg/L

K25mg/L

MgSO4·7H2O125mg/L

KH2PO4125mg/L

Berthelot's液0.5ml/L

Berthelot's液成分组成:

MnSO4·4H2O20g/L

KI0.5g/L

NiCl2·6H200.05g/L

CoCl2·6H200.05g/L

ZnSO4·7H200.10g/L

CuSO4·5H2O0.05g/L

BeSO4·4H2O0.10g/L

H3BO30.05g/L

浓硫酸1ml

2.有机物

维生素B1理学1mg/L

腺嘌呤8mg/L

NAA2mg/L

蔗糖40g／L


锚点四．MS培养基RM—62(Murashige&Skoog(1962))

1.无机盐类同LS培养基

2．有机物




锚点五.花肥培养基Ka963)(Kyotoso1ution)

花肥3g/L

KH2PO4250mg/L

Ca(NO3)2.4H2O1000mg/L

MgSO4·7H2O250mg/L

(NH4)2SO42mg/L

蔗糖20g／L

pH5.0

锚点六．KC培养基KnudsonC(1964)

1.无机盐

KH2PO4250mg/L

Ca(NO3)2.4H2O1000mg/L

(NH4)2SO4500mg/L

MgSO4·7H2O250mg/L

FeSO4·7H2O25mg/L

MnSO4·4H2O7.5mg/L

2.有机物

蔗糖20g／L

pH5.0～5.2


锚点七.Kassanis培养基(1957)

1.无机盐

Ca(NO3)2.4H2O500mg/L

K25mg/L

MgSO4·7H2O125mg/L

KH2PO4125mg/L

Berthelot's液10滴

2.有机物

酪蛋白1mg/L

半胱氨酸10mg/L

腺嘌呤5mg/L

烟酸1mg/L

维生素B61mg/L

泛酸钙10mg/L

肌醇0.1mg/L

维生素H(生物素)0.01mg/L

蔗糖20g／L 锚点八.木本植物用培养基(WPM,WoodyPlantmedium)

1.无机盐

NH4N03400mg／

Ca(NO3)2.4H2O556mg/L

K2SO4990mg/L

CaCl2·2H2O96mg/L

KH2PO4170mg/L

Na2MoO4·2H2O0.25mg/L

MgSO4·7H2O370mg/L

MnSO4·H2O22.4mg/L

ZnSO4·7H2O8.6mg/L

CuSO4·5H2O0.25mg/L

FeSO4·7H2O27.8mg/L

Na2-EDTA37.3mg/L

2.有机物

肌醇100mg/L

维生素B11.0mg/L

烟酸0.5mg/L

维生素B60.5mg/L

甘氨酸2.0mg/L

蔗糖20g／L

琼脂6g／L

pH5.2 锚点九.Nitsch&Nitsch(1969)培养基

1.无机盐

KNO3950mg／L

NH4N03720mg／L

KH2PO468mg/L

CaCl2·2H2O166mg/L

MgSO4·7H2O185mg/L

FeSO4·7H2O27.8mg/L

Na2-EDTA37.3mg/L

MnSO4·4H2O25mg/L

H3BO310mg/L

ZnSO4·7H2O10mg/L

CuSO4·5H2O0.025mg/L

Na2MoO4·2H2O0.25mg/L

2.有机物

维生素H(生物素)0.05mg/L

肌醇100mg/L

维生素B10.5mg/L

烟酸5mg/L

维生素B60.5mg/L

甘氨酸2.0mg/L

叶酸5mg/L

蔗糖20g／L

锚点十.Nielsen(1960)培养基

1.无机盐

KNO3200mg／L

Ca(N03)2·4H2O800mg／L

MgSO4·7H2O200mg/L

KH2PO4200mg/L

ZnSO4·7H2O0.2mg/L

NiCl2·6H2O0.3mg/L

MnCl2·H2O1.8mg/L

CuSO4·5H2O0.08mg/L

H2MoO4·H2O0.02mg/L

H3BO32.8mg/L

2.有机物

维生素B11mg/L

烟酸5mg/L

维生素B61mg/L

蔗糖30g／L 锚点十一.MT(murashgeandTucker)培养基

1.大量元素

NH4N03l650mg／L

K900mg/L

CaCl2·2H2O440mg/L

MgSO4·7H2O370mg/L

KH2PO4170mg/L

Na2一EDTA37.3mg/L

FeSO4·7H2O27.8mg/L

H3BO36.2mg/L

MnSO4·4H2O22.3mg/L

KI0.83mg/L

Na2MoO·2H2O0.25mg/L

CuSO4·5H2O0.025mg/L

CoCl2·6H200.025mg/L

2．有机物

维生素Bl0．4mg/L

维生素B610.0mg/L

烟酸5mg/L

甘氨酸2.0mg/L

肌醇100．0mg／L

蔗糖50g／L

锚点十二.Miller培养基(1963)

1.无机物

NH4N03l000mg／L

K000mg/L

Ca(N03)2·4H2O347mg/L

KH2PO4300mg/L

KCl65mg/L

MgSO4·7H2O35mg/L

Na一Fe一EDTA32mg/L

MnSO4·4H2O4.4mg/L

ZnSO4·7H2O1.5mg/L

H3BO31.6mg/L

KI0.8mg/L

CaCl2·2H2O150mg/L


2．有机物

维生素Bl0.1mg/L

维生素B60.1mg/L

叶酸0.5mg/L

甘氨酸2.0mg/L

蔗糖30g／L

pH6.0


锚点十三.B5培养基(Gamborg等,1968)

1.无机物

NaH2PO4·H2O150mg/L

KNO33000mg/L

(NH4)2SO4134mg／L

MgSO4·7H2O500mg/L

Na2一EDTA37.3mg/L

FeSO4·7H2O27.8mg/L

MnSO4·H2O10mg/L

ZnSO4·7H2O2mg/L

H3BO33mg/L

Na2MoO·2H2O0.25mg/L

CuSO4·5H2O0.025mg/L

CoCl2·6H200.025mg/L

KI10mg/L

2.有机物

维生素B110mg/L

维生素B61mg/L

甘氨酸2.0mg/L

叶酸0.5mg/L

肌醇100．0mg／L

蔗糖50g／L

pH5.5 锚点十四.N6培养基(北京植物所,黑龙江农业科学院,1974)

1.无机物

KNO32800mg/L

(NH4)2SO4463mg／L

KH2PO4400mg/L

MgSO4·7H2O185mg/L

CaCl2·2H2O165mg/L

Na2一EDTA37.3mg/L

FeSO4·7H2O27.8mg/L

MnSO4·H2O4.4mg/L

ZnSO4·7H2O1.5mg/L

H3BO31.6mg/L

KI0.8mg/L

2.有机物

维生素B11mg/L

维生素B60.5mg/L

叶酸1mg/L

蔗糖20g／L

pH5.8 锚点十五.H培养基

1.无机盐

KNO3950mg/L

NH4N03720mg／L

MgSO4·7H2O185mg/L

CaCl2·2H2O166mg/L

KH2PO468mg/L

Na2一EDTA37.3mg/L

FeSO4·7H2O27.8mg/L

MnSO4·4H2O25mg/L

ZnSO4·7H2O10mg/L

H3BO310mg/L

Na2MoO·2H2O0.25mg/L

CuSO4·5H2O0.025mg/L

2.有机物

维生素B10.5mg/L

维生素B60.5mg/L

甘氨酸2.0mg/L

叶酸0.5mg/L

肌醇100．0mg／L

烟酸0.5mg/L

生物素0.05mg/L

蔗糖20g／L

pH5.5

锚点十六.改良White培养基(White,1963)

1.无机盐

KNO380mg/L

Ca(N03)2·4H2O300mg／L

MgSO4·7H2O720mg/L

Na2SO4200mg/L

KCl65mg/L NaH2PO4·5H2O16.5mg/L

Fe(SO4)32.5mg/L

MnSO4·4H2O7mg/L

ZnSO4·7H2O3mg/L

H3BO31.5mg/L

CuSO4·5H2O0.001mg/L

MoO33mg/L 2.有机物

维生素B10.1mg/L

维生素B60.1mg/L

甘氨酸3.0mg/L

肌醇100．0mg／L

烟酸0.3mg/L

蔗糖20g／L

pH5.6